质粒名称: | pY011 (pcDNA3.1-hLb2Cpf1) |
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出品公司: | 爱迪基因 |
目录编号: | 69983 |
存储实验室: | Feng Zhang |
载体骨架基本信息 |
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载体名称: | pcDNA3.1 |
载体抗性: | 氨苄霉素 |
空载体大小: | 5354 bp |
完整质粒大小: | 9400 bp |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | hLb2Cpf1 |
物种来源: | Lachnospiraceae_bacterium_MA2020 |
插入大小: | 3756 bp |
启动子 | CMV |
标签/融合蛋白: | NLS-3xHA(C端) |
克隆方法: | 未知 |
5' 测序引物 | CMV-F |
3' 测序引物 | BGH-rev |
文献引用方法 |
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材料和方法部分: | pY011 (pcDNA3.1-hLb2Cpf1) was a gift from Feng Zhang (Addgene plasmid # 69983) |
参考文献部分: | Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. 10.1016/j.cell.2015.09.038 PubMed 26422227 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pY011 (pcDNA3.1-hLb2Cpf1) Sequencing Result 9164 bp DNA SYN DEFINITION pY011 (pcDNA3.1-hLb2Cpf1) Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..9164 /organism="pY011 (pcDNA3.1-hLb2Cpf1) Sequencing Result" /mol_type="other DNA" promoter 15..631 /label="CMV_immearly_promoter" misc_feature 94..381 /label="CAG_enhancer" misc_feature 548..568 /label="CMV_fwd_primer" promoter 642..660 /label="T7_promoter" CDS 708..4463 /label="ORF frame 3" misc_feature 4380..4406 /label="HA" misc_feature complement(4534..4551) /label="BGH_rev_primer" terminator 4537..4764 /label="bGH_PA_terminator" rep_origin 4827..5133 /label="f1_origin" misc_feature complement(5247..5267) /label="pBABE_3_primer" misc_feature complement(5253..5468) /label="SV40_enhancer" promoter 5265..5533 /label="SV40_promoter" rep_origin 5432..5509 /label="SV40_origin" misc_feature 5494..5513 /label="SV40pro_F_primer" CDS 5648..6442 /label="ORF frame 2" gene 5651..6439 /label="NeoR/KanR" /gene="NeoR/KanR" CDS complement(5957..6493) /label="ORF frame 2" terminator 6619..6738 /label="SV40_PA_terminator" misc_feature 6707..6726 /label="EBV_rev_primer" promoter complement(6782..6800) /label="M13_reverse_primer" misc_feature complement(6799..6821) /label="M13_pUC_rev_primer" promoter complement(6835..6864) /label="lac_promoter" gene complement(7947..8807) /label="Ampicillin" /gene="Ampicillin" CDS complement(7947..8807) /label="ORF frame 1" promoter complement(8849..8877) /label="AmpR_promoter" ORIGIN 1 AGATATACGC GTTGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA 61 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT 121 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA 181 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC 241 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT 301 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG 361 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT 421 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT 481 GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC 541 CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCTC 601 TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC TCACTATAGG 661 GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CGCCACCATG TACTATGAGT 721 CCCTGACCAA GCAGTACCCC GTGTCTAAGA CAATCCGGAA TGAGCTGATC CCTATCGGCA 781 AGACACTGGA TAACATCCGC CAGAACAATA TCCTGGAGAG CGACGTGAAG CGGAAGCAGA 841 ACTACGAGCA CGTGAAGGGC ATCCTGGATG AGTATCACAA GCAGCTGATC AACGAGGCCC 901 TGGACAATTG CACCCTGCCA TCCCTGAAGA TCGCCGCCGA GATCTACCTG AAGAATCAGA 961 AGGAGGTGTC TGACAGAGAG GATTTCAACA AGACACAGGA CCTGCTGAGG AAGGAGGTGG 1021 TGGAGAAGCT GAAGGCCCAC GAGAACTTTA CCAAGATCGG CAAGAAGGAC ATCCTGGATC 1081 TGCTGGAGAA GCTGCCTTCC ATCTCTGAGG ACGATTACAA TGCCCTGGAG AGCTTCCGCA 1141 ACTTTTACAC CTATTTCACA TCCTACAACA AGGTGCGGGA GAATCTGTAT TCTGATAAGG 1201 AGAAGAGCTC CACAGTGGCC TACAGACTGA TCAACGAGAA TTTCCCAAAG TTTCTGGACA 1261 ATGTGAAGAG CTATAGGTTT GTGAAAACCG CAGGCATCCT GGCAGATGGC CTGGGAGAGG 1321 AGGAGCAGGA CTCCCTGTTC ATCGTGGAGA CATTCAACAA GACCCTGACA CAGGACGGCA 1381 TCGATACCTA CAATTCTCAA GTGGGCAAGA TCAACTCTAG CATCAATCTG TATAACCAGA 1441 AGAATCAGAA GGCCAATGGC TTCAGAAAGA TCCCCAAGAT GAAGATGCTG TATAAGCAGA 1501 TCCTGTCCGA TAGGGAGGAG TCTTTCATCG ACGAGTTTCA GAGCGATGAG GTGCTGATCG 1561 ACAACGTGGA GTCTTATGGC AGCGTGCTGA TCGAGTCTCT GAAGTCCTCT AAGGTGAGCG 1621 CCTTCTTTGA TGCCCTGAGA GAGTCTAAGG GCAAGAACGT GTACGTGAAG AATGACCTGG 1681 CCAAGACAGC CATGAGCAAC ATCGTGTTCG AGAATTGGAG GACCTTTGAC GATCTGCTGA 1741 ACCAGGAGTA CGACCTGGCC AACGAGAACA AGAAGAAGGA CGATAAGTAT TTCGAGAAGC 1801 GCCAGAAGGA GCTGAAGAAG AATAAGAGCT ACTCCCTGGA GCACCTGTGC AACCTGTCCG 1861 AGGATTCTTG TAACCTGATC GAGAATTATA TCCACCAGAT CTCCGACGAT ATCGAGAATA 1921 TCATCATCAA CAATGAGACA TTCCTGCGCA TCGTGATCAA TGAGCACGAC AGGTCCCGCA 1981 AGCTGGCCAA GAACCGGAAG GCCGTGAAGG CCATCAAGGA CTTTCTGGAT TCTATCAAGG 2041 TGCTGGAGCG GGAGCTGAAG CTGATCAACA GCTCCGGCCA GGAGCTGGAG AAGGATCTGA 2101 TCGTGTACTC TGCCCACGAG GAGCTGCTGG TGGAGCTGAA GCAGGTGGAC AGCCTGTATA 2161 ACATGACCAG AAATTATCTG ACAAAGAAGC CTTTCTCTAC CGAGAAGGTG AAGCTGAACT 2221 TTAATCGCAG CACACTGCTG AACGGCTGGG ATCGGAATAA GGAGACAGAC AACCTGGGCG 2281 TGCTGCTGCT GAAGGACGGC AAGTACTATC TGGGCATCAT GAACACAAGC GCCAATAAGG 2341 CCTTCGTGAA TCCCCCTGTG GCCAAGACCG AGAAGGTGTT TAAGAAGGTG GATTACAAGC 2401 TGCTGCCAGT GCCCAACCAG ATGCTGCCAA AGGTGTTCTT TGCCAAGAGC AATATCGACT 2461 TCTATAACCC CTCTAGCGAG ATCTACTCCA ATTATAAGAA GGGCACCCAC AAGAAGGGCA 2521 ATATGTTTTC CCTGGAGGAT TGTCACAACC TGATCGACTT CTTTAAGGAG TCTATCAGCA 2581 AGCACGAGGA CTGGAGCAAG TTCGGCTTTA AGTTCAGCGA TACAGCCTCC TACAACGACA 2641 TCTCCGAGTT CTATCGCGAG GTGGAGAAGC AGGGCTACAA GCTGACCTAT ACAGACATCG 2701 ATGAGACATA CATCAATGAT CTGATCGAGC GGAACGAGCT GTACCTGTTC CAGATCTATA 2761 ATAAGGACTT TAGCATGTAC TCCAAGGGCA AGCTGAACCT GCACACACTG TATTTCATGA 2821 TGCTGTTTGA TCAGCGCAAT ATCGACGACG TGGTGTATAA GCTGAACGGA GAGGCAGAGG 2881 TGTTCTATAG GCCAGCCTCC ATCTCTGAGG ACGAGCTGAT CATCCACAAG GCCGGCGAGG 2941 AGATCAAGAA CAAGAATCCT AACCGGGCCA GAACCAAGGA GACAAGCACC TTCAGCTACG 3001 ACATCGTGAA GGATAAGCGG TATAGCAAGG ATAAGTTTAC CCTGCACATC CCCATCACAA 3061 TGAACTTCGG CGTGGATGAG GTGAAGCGGT TCAACGACGC CGTGAACAGC GCCATCCGGA 3121 TCGATGAGAA TGTGAACGTG ATCGGCATCG ACCGGGGCGA GAGAAATCTG CTGTACGTGG 3181 TGGTCATCGA CTCTAAGGGC AACATCCTGG AGCAGATCTC CCTGAACTCT ATCATCAATA 3241 AGGAGTACGA CATCGAGACA GATTATCACG CACTGCTGGA TGAGAGGGAG GGCGGCAGAG 3301 ATAAGGCCCG GAAGGACTGG AACACCGTGG AGAATATCAG GGACCTGAAG GCCGGCTACC 3361 TGAGCCAGGT GGTGAACGTG GTGGCCAAGC TGGTGCTGAA GTATAATGCC ATCATCTGCC 3421 TGGAGGACCT GAACTTTGGC TTCAAGAGGG GCCGCCAGAA GGTGGAGAAG CAGGTGTACC 3481 AGAAGTTCGA GAAGATGCTG ATCGATAAGC TGAATTACCT GGTCATCGAC AAGAGCCGCG 3541 AGCAGACATC CCCTAAGGAG CTGGGAGGCG CCCTGAACGC ACTGCAGCTG ACCTCTAAGT 3601 TCAAGAGCTT TAAGGAGCTG GGCAAGCAGT CCGGCGTGAT CTACTATGTG CCTGCCTACC 3661 TGACCTCTAA GATCGATCCA ACCACAGGCT TCGCCAATCT GTTTTATATG AAGTGTGAGA 3721 ACGTGGAGAA GTCCAAGAGA TTCTTTGACG GCTTTGATTT CATCAGGTTC AACGCCCTGG 3781 AGAACGTGTT CGAGTTCGGC TTTGACTACC GGAGCTTCAC CCAGAGGGCC TGCGGCATCA 3841 ATTCCAAGTG GACCGTGTGC ACCAACGGCG AGCGCATCAT CAAGTATCGG AATCCAGATA 3901 AGAACAATAT GTTCGACGAG AAGGTGGTGG TGGTGACCGA TGAGATGAAG AACCTGTTTG 3961 AGCAGTACAA GATCCCCTAT GAGGATGGCA GAAATGTGAA GGACATGATC ATCAGCAACG 4021 AGGAGGCCGA GTTCTACCGG AGACTGTATA GGCTGCTGCA GCAGACCCTG CAGATGAGAA 4081 ACAGCACCTC CGACGGCACA AGGGATTACA TCATCTCCCC TGTGAAGAAT AAGAGAGAGG 4141 CCTACTTCAA CAGCGAGCTG TCCGACGGCT CTGTGCCAAA GGACGCCGAT GCCAACGGCG 4201 CCTACAATAT CGCCAGAAAG GGCCTGTGGG TGCTGGAGCA GATCAGGCAG AAGAGCGAGG 4261 GCGAGAAGAT CAATCTGGCC ATGACCAACG CCGAGTGGCT GGAGTATGCC CAGACACACC 4321 TGCTGAAAAG GCCGGCGGCC ACGAAAAAGG CCGGCCAGGC AAAAAAGAAA AAGGGATCCT 4381 ACCCATACGA TGTTCCAGAT TACGCTTATC CCTACGACGT GCCTGATTAT GCATACCCAT 4441 ATGATGTCCC CGACTATGCC TAAGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG 4501 AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC 4561 CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG 4621 TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC 4681 TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG 4741 CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG 4801 GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 4861 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 4921 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 4981 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 5041 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 5101 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 5161 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 5221 AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC 5281 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG 5341 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC 5401 AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC 5461 CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC 5521 TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 5581 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC 5641 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 5701 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 5761 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 5821 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 5881 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 5941 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 6001 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 6061 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 6121 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 6181 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 6241 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 6301 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 6361 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 6421 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG 6481 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC 6541 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG 6601 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC 6661 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA 6721 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA 6781 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA 6841 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA 6901 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGcattaa 6961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctCTTC CGCTTCCTCG 7021 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 7081 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 7141 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 7201 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 7261 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 7321 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 7381 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 7441 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 7501 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 7561 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 7621 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 7681 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 7741 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 7801 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 7861 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 7921 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 7981 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 8041 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 8101 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 8161 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 8221 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 8281 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 8341 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 8401 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 8461 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 8521 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 8581 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 8641 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 8701 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 8761 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 8821 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 8881 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 8941 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 9001 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 9061 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 9121 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCC //